Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles
Identifieur interne : 000699 ( new/Analysis ); précédent : 000698; suivant : 000700Surface-displayed porcine reproductive and respiratory syndrome virus from cell culture onto gram-positive enhancer matrix particles
Auteurs : Lan Li ; Xuwen Qiao ; Jin Chen ; Yuanpeng Zhang ; Qisheng Zheng ; Jibo HouSource :
- Journal of Industrial Microbiology & Biotechnology [ 1367-5435 ] ; 2018.
Abstract
Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in
Url:
DOI: 10.1007/s10295-018-2061-1
PubMed: 30046953
PubMed Central: 7088258
Affiliations:
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PMC:7088258Le document en format XML
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Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Chen, Jin" sort="Chen, Jin" uniqKey="Chen J" first="Jin" last="Chen">Jin Chen</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Zhang, Yuanpeng" sort="Zhang, Yuanpeng" uniqKey="Zhang Y" first="Yuanpeng" last="Zhang">Yuanpeng Zhang</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation></author><author><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0017 5204</institution-id><institution-id institution-id-type="GRID">grid.454840.9</institution-id><institution>National Research, Center of Engineering and Technology for Veterinary Biologicals,</institution><institution>Jiangsu Academy of Agricultural Sciences,</institution></institution-wrap>Nanjing, 210014 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">210014 Jiangsu China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2">Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 Jiangsu China</nlm:aff><wicri:noCountry code="subfield">225009 Jiangsu China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Journal of Industrial Microbiology & Biotechnology</title><idno type="ISSN">1367-5435</idno><idno type="eISSN">1476-5535</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Vaccine immunization is now one of the most effective ways to control porcine reproductive and respiratory syndrome virus (PRRSV) infection. Impurity is one of the main factors affecting vaccine safety and efficacy. Here we present a novel innovative PRRSV purification approach based on surface display technology. First, a bifunctional protein PA-GRFT (protein anchor-griffithsin), the crucial factor in the purification process, was successfully produced in <italic>Escherichia coli</italic> yielding 80 mg/L of broth culture. Then PRRSV purification was performed by incubation of PA-GRFT with PRRSV and gram-positive enhancer matrix (GEM) particles, followed by centrifugation to collect virions loaded onto GEM particles. Our results showed that most of the bulk impurities had been removed, and PA-GRFT could capture PRRSV onto GEM particles. Our lactic acid bacteria-based purification method, which is promising as ease of operation, low cost and easy to scale-up, may represent a candidate method for the large-scale purification of this virus for vaccine production.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bohua, L" uniqKey="Bohua L">L Bohua</name></author><author><name sortKey="Ming, S" uniqKey="Ming S">S Ming</name></author><author><name sortKey="Lu, Y" uniqKey="Lu Y">Y Lu</name></author><author><name sortKey="Xiaoyu, D" uniqKey="Xiaoyu D">D Xiaoyu</name></author><author><name sortKey="Baochun, L" uniqKey="Baochun L">L Baochun</name></author><author><name sortKey="Fenqin, S" uniqKey="Fenqin S">S Fenqin</name></author><author><name sortKey="Li, Z" uniqKey="Li Z">Z Li</name></author><author><name sortKey="Xizhao, C" uniqKey="Xizhao C">C Xizhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bosma, T" uniqKey="Bosma T">T 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last="Chen">Jin Chen</name><name sortKey="Hou, Jibo" sort="Hou, Jibo" uniqKey="Hou J" first="Jibo" last="Hou">Jibo Hou</name><name sortKey="Li, Lan" sort="Li, Lan" uniqKey="Li L" first="Lan" last="Li">Lan Li</name><name sortKey="Qiao, Xuwen" sort="Qiao, Xuwen" uniqKey="Qiao X" first="Xuwen" last="Qiao">Xuwen Qiao</name><name sortKey="Zhang, Yuanpeng" sort="Zhang, Yuanpeng" uniqKey="Zhang Y" first="Yuanpeng" last="Zhang">Yuanpeng Zhang</name><name sortKey="Zheng, Qisheng" sort="Zheng, Qisheng" uniqKey="Zheng Q" first="Qisheng" last="Zheng">Qisheng Zheng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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